The Tennessee Wildlife Resources Agency (also known as TWRA) is an independent state agency of the state of Tennessee with the mission of managing the state's fish and wildlife and their habitats, as well as responsibility for all wildlife related law enforcement activities. The agency also has responsibility for fostering the safe use of the state's waters through a program of law enforcement, education, and access.

Law enforcement
The TWRA takes an active role in wildlife and fisheries conservation and the reintroduction of wildlife that were driven from an area due to human intervention. Recent conservation activities included the successful reintroduction of wild turkeys to West and Middle Tennessee, as well as a successful elk reintroduction program in East Tennessee. The TWRA manages over 215,000 acres (870 km²) of forested land for public hunting and wildlife research. The TWRA maintains a state of the art forensics laboratory that includes state of the art DNA analysis equipment at the University of Tennessee, Martin.
TWRA officers are often called to provide assistance in search and rescue operations, due to their extensive experience working in woodland environments. TWRA officers also are included in Department of Homeland Security training as first responders and anti-terrorism enforcement officials.
TWRA officers were recently sent to the Gulf Coast to provide assistance to local law enforcement and rescue teams in the aftermath of Hurricane Katrina.